Instruments

Q Exactive: Staff Operated

Thermo Scientific Q ExactiveTM HF Hybrid Quadrupole-OrbitrapTM Mass Spectrometer combines a segmented quadrupole for high-performance precursor ion selection with a high-resolution, accurate-mass (HR/AM) ultra-high-field Orbitrap mass analyzer to deliver a superior combination of scan speed, resolving power, mass accuracy, spectral quality and sensitivity. Additionally, has Intact Protein Mode for characterizing intact proteins.

Thermo Scientific Q Exactive HF Hybrid Quadrupole-Orbitrap Mass Spectrometer combines a segmented quadrupole for high-performance precursor ion selection with a high-resolution, accurate-mass (HR/AM) ultra-high-field Orbitrap mass analyzer to deliver a superior combination of scan speed, resolving power, mass accuracy, spectral quality and sensitivity. In addition, has Intact Protein Mode for characterizing intact proteins. 

LTQ Orbitrap: Staff Operated

Thermo LTQ Orbitrap Velos HPLC-nESI-LIT-Orbitrap is a very high-resolution electrospray tandem mass spectrometer that helps researchers identify proteins and other molecules.

MALDI-TOF/TOF: Staff Operated

 AB Sciex 5800 MALDI-TOF/TOF is a tandem mass spectrometer used to identify and quantify proteins and also map the location of molecules. The ability to map molecules within an intact tissue sample – termed Mass Spectrometry Imaging – complements traditional microscopic techniques. 

Xevo TQ-S: Staff Operated

  

Waters Xevo TQ-S UPLC-nESI-Triple Quadrupole is a tandem mass spectrometer that can be used to validate potential biomarkers. The TQ-S can be connected to The Waters Atmospheric Pressure GC/MS with Agilent 7693 Autosampler to analyze volatile and semi-volatile compounds or to the Waters NanoAcquity Ultra Performance Liquid Chromatograph, which uses low flow rates (nL/min) and high pressures (5000-6000 psi) to separate small quantities of proteins. 

MALDI-TOF: Researcher Operated

Shimadzu Axima CFR MALDI-TOF is a time-of-flight mass spectrometer with both linear and reflectron operational modes. The instrument uses matrix-assisted laser desorption/ionization as ionization source. The mass range exceeds 100KDa in the linear mode and 10KDa in the reflectron mode. This instrument allows researchers to learn how to operate a mass spectrometer and identify proteins in their samples. 

LTQ: Researcher Operated

Thermo LTQ HPLC-nESI-LIT is a tandem mass spectrometer that allows researchers to learn how to operate a mass spectrometer and identify proteins in samples.
 

 

HiSeq 2500: Staff Operated

Illumina HiSeq 2500 Sequencer is a next-generation sequencer used to determine the sequence of entire genomes (DNA), exomes (Exon), transcriptomes (RNA), miRNomes (miRNA), Cistromes (ChIP-Seq) extremely quickly. It’s capable of repeating the human genome project – which took seven years and cost $3 billion – in two days for less than $2,000. 

Covaris: Researcher Operated

Covaris E220 Ultrasonicator is capable of rapid and complete homogenization, tissue disruption and sample extraction. In addition, it can be used for DNA or chromatin fragmentation in sample preparation for the HiSeq 2500 sequencer.

 

OFFGEL Fractionator: Researcher Operated

Agilent OFFGEL Fractionator separates proteins according to the pH at which they have no net charge, allowing researchers to simplify protein mixtures before mass spec analysis. 

Differential Scanning Calorimeter: Researcher Operated

TA Instruments nano-Differential Scanning Calorimeter can measure extremely small heat flows, thereby determining the change in the stability of a protein when it interacts with other molecules.

HPLC: Researcher Operated

Agilent 1260 HPLC with Wyatt Dawn HELEOS light-scattering detector is a high-performance liquid chromatograph equipped with a light-scattering detector. It allows researchers to separate proteins from mixtures and measure their size and weight to help them assess the proteins’ solubility and purity. 

FPLC: Researcher Operated

GE Healthcare/Amersham Akta FPLC is a liquid chromatograph for purification of larger quantities of proteins. 

SpeedVac: Researcher Operated

Thermo Scientific Express SC250EXP SpeedVac™ Concentrator System uses centrifugation, vacuum and heat technologies for removing solvents and concentrating samples.

 

Bioanalyzer: Researcher Operated

Agilent 2100 Bioanalyzer is a microfluidic electrophoresis system used to measure the quantity, quality and average size of DNA, RNA or protein in a sample. Accurate sizing of samples intended for sequencing on the HiSeq 2500 can be accomplished with the Bioanalyzer. 

Qubit: Researcher Operated

 

Thermo Scientific Qubit 2.0 is a benchtop fluorometer for quantitation of DNA (dsDNA & ssDNA), RNA and protein. Target-specific fluorescent dyes are used to minimize quantitation of contaminating molecules. This system is more accurate for samples with low quantity particularly for sequencing on the HiSeq 2500. 

NanoVue: Researcher Operated

GE Healthcare NanoVue is a UV/Vis spectrophotometer that measures nucleic acids and proteins with sample volumes as low as 0.5 microliters. Measures: DNA, RNA, Oligonucleotides, Tm Calculation, Cy Dye DNA probe labeling, Protein UV & A280, BCA, Bradford, Lowry, & Biuret. 

Spectrophotometer: Researcher Operated

Agilent Cary 5000 UV/Vis/NIR is a spectrophotometer that allows researchers to determine the presence and quantity of chemical classes in a solution and for measuring the amounts of protein or DNA in a sample.