Bioinformatics information for Illumina Hiseq 2500 sequencing data:
In order to access the sequencing data, the user must have a valid FSU Research Computing Center (RCC) account. RCC Account Signup.
If sequencing samples are pooled and demultiplexing is needed, the user should provide an index sheet to Dr. Yanming Yang (email@example.com) by following the example format (IndexSheetExample.csv).
Note: The index sheet format and headers are strictly enforced by the demultiplexing software. You may leave FCID and Recipe columns blank. The space character and the other following characters are not allowed: ? ( ) [ ] / \ = + < > : ; " ' , * ^ | &
FSU RCC preferred approach for data transfer is via Globus.
Please use the following information on the Globus transfer page:
Path to data: /lustre/medicine/sequencer/Outputs_CASAVA/
The following software packages are available for data analysis at COM spear nodes (spear-17, 18, 19, 20, and 22): Samtools, Biopython, Abyss, Bowtie/Bowtie2, BWA, Tophat, Cufflinks, R packages, Trimmomatic. These and other software packages are available for general access at the RCC.