NovaSeq Data Access/Analysis

Information for Illumina NovaSeq X Plus sequencing data:

RCC Account:

In order to access the sequencing data, generate an account (guest account if outside FSU) with the FSU Research Computing Center (RCC).

Demultiplexing:

If sequencing samples are pooled and demultiplexing is needed, the user should provide an index sheet to TSL (yanming.yang@med.fsu.edu; cynthia.vied@med.fsu.edu).  The index sheet should be generated using the Illumina Experiment Manager software for demultiplexing compatibility.

Data Access:
First, login to FSU RCC: YOUR_RCC_ID@hpc-login.rcc.fsu.edu.
Then, follow the provided path in the data releasing e-mail.
Data Transfer:

FSU RCC preferred approach for data transfer is via Globus.

Please use the following information on the Globus transfer page:
             Endpoint: fsurcc#GPFS#research
            Path to data: /medicine/sequencer/CORRESPONDING_PATH_PROVIDED_BY_EMAIL

Analysis Software:

The following software packages and others are available for data analysis at the RCC: FastQC, Samtools, Biopython, Abyss, Bowtie/Bowtie2, BWA, Star aligner, Tophat, Cufflinks, R packages, HTSeq, Trimmomatic.