Bioinformatics information for Illumina NovaSeq 6000 sequencing data:
In order to access the sequencing data, login to the FSU Research Computing Center (RCC) and request access to medicine nodes or generate a guest account if outside FSU. RCC Account Signup.
If sequencing samples are pooled and demultiplexing is needed, the user should provide an index sheet to TSL (firstname.lastname@example.org; email@example.com). The index sheet should be generated using the Illumina Experiment Manager software for demultiplexing compatibility.
FSU RCC preferred approach for data transfer is via Globus.
Please use the following information on the Globus transfer page:
Path to data: THE_PATH_RELEASED_VIA_EMAIL
The following software packages are available for data analysis at COM spear nodes (spear-17, 18, 19, 20, and 22): Samtools, Biopython, Abyss, Bowtie/Bowtie2, BWA, Tophat, Cufflinks, R packages, Trimmomatic. These and other software packages are available for general access at the RCC.