Bioinformatics Resources

Bioinformatics information for Illumina NovaSeq 6000 sequencing data:

RCC Account:

In order to access the sequencing data, login to the FSU Research Computing Center (RCC) and request access to medicine nodes or generate a guest account if outside FSU. RCC Account Signup.

Demultiplexing:

If sequencing samples are pooled and demultiplexing is needed, the user should provide an index sheet to TSL (yanming.yang@med.fsu.edu; cynthia.vied@med.fsu.edu).  The index sheet should be generated using the Illumina Experiment Manager software for demultiplexing compatibility.

Data Access:
First, login to FSU RCC: YOUR_RCC_ID@hpc-login.rcc.fsu.edu.
Then, get to a spear node to manipulate your data:
Either:
ssh YOUR_RCC_ID@spear-X (X = 1,2,... 8) into general spear nodes (more powerful)
Or:
ssh YOUR_RCC_ID@spear-Y (Y = 17, 18, 19, 20, or 22) into medicine spear nodes
 
Data Transfer:

FSU RCC preferred approach for data transfer is via Globus.

Please use the following information on the Globus transfer page:
             Endpoint: fsurcc#gpfs
             Path to data: THE_PATH_RELEASED_VIA_EMAIL

Analysis Software:

The following software packages are available for data analysis at COM spear nodes (spear-17, 18, 19, 20, and 22): Samtools, Biopython, Abyss, Bowtie/Bowtie2, BWA, Tophat, Cufflinks, R packages, Trimmomatic. These and other software packages are available for general access at the RCC