Flow Cytometry Laboratory

The FSU College of Medicine Flow Cytometry Core Laboratory offers cutting-edge Aurora analyzer, Aurora CS sorter and Amnis ImageStream from Cytek to cover your flow cytometry needs. 

Both Auroras offer full spectrum technology and auto fluorescence extraction in SpectroFlo software. Each is equipped with 5 lasers (355nm, 405nm, 488nm, 561nm, 640nm) and total 64 APD detectors enable more efficient spectrum capture for dyes emitting in the 365-829 nm range. Spectral unmixing feature allows the use of two dyes with similar peak emissions.

ImageStream Mk II Imaging Flow Cytometer (ISX) designed for the rapid acquisition of millions of cells with up to 12 channels of cellular imagery. Combined with IDEA software where each object is measured for hundreds of parameters using not only fluorescence intensity but the fluorescence spatial location.   

To obtain the best data, please consult with core staff prior to beginning your experiments.  Training on the Aurora and ISX is also provided by core staff. 

 

Image shows a line drawing of the steps of the flow cytometry process including preparation, staining, sorting, and analysis of samples.

 

Office Contacts

For more information, contact:
Li Sun
Director
Phone: 850-368-0169