Resources

Applications:

  • Cell marker profiling: With the advanced full spectrum profiling (FSP), up to 40 colors can be detected simultaneously.

  • Cell Sorting (FACS): Isolating specific populations (e.g., stem cells) for downstream culture or single-cell sequencing.

  • Cell Cycle & DNA Analysis: Measuring G1, S, and G2/M phases using dyes like Propidium Iodide or DAPI.

  • Apoptosis Assays: Distinguishing between programmed cell death and necrosis using Annexin V and 7-AAD.

  • Subcellular/colocalization Assay (ISX only): Cell images acquired by microscope from the flow cell were used for spatial information analysis.

  • Cytokine Profiling: Beads based ELISA enable profiling up to 14 soluble cytokines from 50 µl sample. BioLegend

  • Metabolic Profiling: Measuring protein synthesis as a proxy for metabolic state (glycolysis vs. oxidative phosphorylation) at the single-cell level. SCENITH

  • Extracellular Vesicle (EV) Analysis: Characterizing exosomes and microvesicles (100-1000 nm) for liquid biopsies. MIFlowCyt-EV

  • Phosflow: Measuring intracellular signaling by staining for phosphorylated proteins (e.g., p-STAT, p-ERK). BD

  • And more…

 

Training and Protocols:

  • Flow cytometry basic and Antibody panel design Cytek

  • General cell staining protocols (surface and intracellular) BioLegend

 

Instrument User Manual:

Office Contacts

For more information, contact:
Li Sun
Director
Phone: 850-368-0169