Publications

2019

Johnston, J.R., Landim-Vieira, M., Marques, M.A., de Oliveira, G.A.P., Gonzalez-Martinez, D., Moraes, A.H., He, H., Iqbal, A., Wilnai, Y., Birk, E., Zucker, N., Silva, J.L., Chase, P.B., Pinto, J.R. The intrinsically disordered carboxy-terminus of troponin-T binds to troponin-C to modulate myocardial force generation. J Biol Chem. 2019 (In press). doi:10.1074/jbc.RA119.011177

Johnson, D., Zhu, L., Landim-Vieira, M., Pinto, J.R., Chalovich, J.M. Basic residues within the cardiac troponin T C-terminus are required for full inhibition of muscle contraction and limit activation by calcium. J Biol Chem. 2019 Nov 11 (In press). doi:10.1074/jbc.RA119.010966

Landim-Vieira, M., Schipper, J.M., Pinto, J.R., Chase, P.B. Cardiomyocyte nuclearity and ploidy: when is double trouble? J Muscle Res Cell Motil. 2019 Jul 17. doi: 10.1007/s10974-019-09545-7. 

Parvatiyar, M.S., Brownstein, A.J., Kanashiro-Takeuchi, R.M., Collado, J.R., Dieseldorff Jones, K.M., Gopal, J., Hammond, K.G., Marshall, J.L., Ferrel, A., Beedle, A.M., Chamberlain, J.S., Pinto, J.R., Crosbie, R.H. Stabilization of the cardiac sarcolemma by sarcospan rescues DMD-associated cardiomyopathy. JCI Insight. Apr 30;5, 2019. doi: 10.1172/jci.insight.123855.

Marques, M.A., Parvatiyar, M.S., Yang, W., de Oliveira, G.A.P., Pinto, J.R. The missing links within troponin. Arch Biochem Biophys. 663:95-100, 2019. doi: 10.1016/j.abb.2018.12.026.

Dieseldorff Jones, K.M., Koh, Y., Weller, R.S., Turna, R.S., Ahmad, F., Huke, S., Knollmann, B.C., Pinto, J.R., Hwang, H.S. Pathogenic troponin T mutants with opposing effects on myofilament Ca2+ sensitivity attenuate cardiomyopathy phenotypes in mice. Arch Biochem Biophys. 661:125-131, 2019. doi: 10.1016/j.abb.2018.11.006.

2018

Gonzalez-Martinez, D., Johnston. J.R., Landim-Vieira, M., Ma, W., Antipova, O., Awan, O., Irving, T.C., Chase, P.B., Pinto, J.R. Structural and functional impact of troponin C-mediated Ca2+ sensitization on myofilament lattice spacing and cross-bridge mechanics in mouse cardiac muscle. J Mol Cell Cardiol. 123:26-37, 2018. doi: 10.1016/j.yjmcc.2018.08.015.

Serra, A.J., Prokic, M.D., Vasconsuelo, A., Pinto, J.R. Oxidative Stress in Muscle Diseases: Current and Future Therapy. Oxid Med Cell Longev. 26; 2018:6439138, 2018. doi: 10.1155/2018/6439138. eCollection 2018.

Johnston, J.R., Chase, P.B., Pinto, J.R. Troponin through the looking-glass: emerging roles beyond regulation of striated muscle contraction. Oncotarget, 9(1): 1461-1482, 2018. https://doi.org/10.18632/oncotarget.22879.

Wang, L., Kim, K., Parikh, S., Cadar, A.G., Bersell, K.R., He, H., Pinto, J.R., Kryshtal, D.O., Knollmann, B,C. Hypertrophic cardiomyopathy-linked mutation in troponin T causes myofibrillar disarray and pro-arrhythmic action potential changes in human iPSC cardiomyocytes. J Mol Cell Cardiol. 114:320-327, 2018. doi: 10.1016/j.yjmcc.2017.12.002.

2017

Wang, L., Kryshtal, D.O., Kim, K., Parikh, S., Cadar, A.G., Bersell, K.R., He, H., Pinto, J.R., Knollmann, B.C. Myofilament Calcium-Buffering Dependent Action Potential Triangulation in Human-Induced Pluripotent Stem Cell Model of Hypertrophic Cardiomyopathy. J Am Coll Cardiol. 70(20):2600-2602, 2017. doi: 10.1016/j.jacc.2017.09.033.

Baxley, T., Johnson, D., Pinto, J.R., Chalovich, J.M. Troponin C mutations partially stabilize the active state of regulated actin and fully stabilize the active state when paired with D14 TnT. Biochemistry 56(23): 2928-2937, 2017. doi: 10.1021/acs.biochem.6b01092.

Bollen, I.A.E., Schuldt, M., Harakalova, M., Vink, A., Asselbergs, F.W., Pinto, J.R., Krüger, M., Kuster, D.W.D., Van der Velden, J. Genotype-specific pathogenic effects in human dilated cardiomyopathy. J. Physiol. 595(14): 4677-4693, 2017. doi: 10.1113/JP274145.

Dossat, A.M., Sanchez-Gonzalez, M.A., Koutnik, A.P., Leitner, S., Ruiz, E.L., Griffin, B., Rosenberg, J.T., Grant, S.C., Fincham, F.D., Pinto, J.R., Kabbaj, M. Pathogenesis of depression- and anxiety-like behavior in an animal model of hypertrophic cardiomyopathy. FASEB J. 31(6): 2492-2506, 2017. doi: 10.1096/fj.201600955RR.

Kawai, M., Johnston, J.R., Karam, T., Wang, L., Singh, R.K., Pinto, J.R. Myosin Rod Hypophosphorylation and CB Kinetics in Papillary Muscles from a TnC-A8V KI Mouse Model. Biophys. J. (London) 112(8): 1726-1736, 2017. doi: http://dx.doi.org/10.1016/j.bpj.2017.02.045

Veltri, T., Landim-Vieira, M., Parvatiyar, M.S., Gonzalez-Martinez, D., Dieseldorff Jones, K.M., Michell, C.A., Dweck, D., Landstrom, A.P., Chase, P.B., Pinto, J.R. Hypertrophic cardiomyopathy cardiac Troponin C mutations defferentially affect slow skeletal and cardiac muscle regulation. Front. Physiol. April(8): 221, 2017. doi: 10.3389/fphys.2017.00221.

Veltri, T., de Oliveira, G.A., Bienkiewicz, E.A., Palhano, F.L., Marques, M.A., Moraes, A.H., Silva, J.L., Sorenson, M.M., Pinto, J.R. Amide hydrogens reveal a temperature-dependent structural transition that enhances site-II Ca2+-binding affinity in a C-domain mutant of cardiac troponin C. Sci Rep. Apr 6;7(1):691, 2017. doi: 10.1038/s41598-017-00777-6.

Pinto, J.R., Muller-Delp, J., Chase, P.B. Will you still need me (Ca2+ , TnT, and DHPR), will you still cleave me (calpain), when I'm 64? Aging Cell. Apr;16(2):202-204, 2017. doi: 10.1111/acel.12560

Marques, M.A., Pinto, J.R., Moraes, A.H., Iqbal, A., de Magalhães, M.T., Monteiro, J., Pedrote, M.M., Sorenson, M.M., Silva, J.L., de Oliveira, G.A. Allosteric transmission along a loosely structured backbone allows a cardiac Troponin C mutant to function with only one Ca2+ ion. J Biol Chem. Feb 10;292(6):2379-2394, 2017. doi: 10.1074/jbc.M116.765362.

2016

Badr, M.A., Pinto, J.R., Davidson, M.W., Chase, P.B. Fluorescent Protein-Based Ca2+ Sensor Reveals Global, Divalent Cation-Dependent Conformational Changes in Cardiac Troponin C. PLoS One. Oct 13;11(10):e0164222, 2016. doi: 10.1371/journal.pone.0164222

Sheng, J.J., Feng, HZ., Pinto, J.R., Wei, H., Jin, J.P. Increases of desmin and a-actinin in mouse cardiac myofibrils as a response to diastolic dysfunction. J Mol Cell Cardiol. Oct;99:218-229, 2016. doi: 10.1016/j.yjmcc.2015.10.035

Na, I., Kong, M.J., Straight, S., Pinto, J.R., Uversky, V.N. Troponins, intrinsic disorder, and cardiomyopathy. Biol Chem. Aug 1;397(8):731-51, 2016. doi: 10.1515/hsz-2015-0303

Zot, H.G., Hasbun, J.E., Michell, C.A., Landim-Vieira, M., Pinto, J.R. Enhanced troponin I binding explains the functional changes produced by the hypertrophic cardiomyopathy mutation A8V of cardiac troponin C. Arch Biochem Biophys. Jul 1;601:97-104, 2016. doi: 10.1016/j.abb.2016.03.011

2015

Figueiredo-Freitas, C., Dulce, R., Foster, M.W., Liang, J., Yamashita, A.M., Lima-Rosa, F.L., Thompson, W.J., Moseley, A.M., Hare, J.M., Nogueira, L., Sorenson, M.M., Pinto, J.R. S-nitrosylation of sarcomeric proteins depresses myofilament Ca2+ sensitivity in intact cardiomyocytes. Antioxid Redox Signal. Nov 1;23(13):1017-34, 2015. doi: 10.1089/ars.2015.6275

Martins, A.S., Parvatiyar, M.S., Feng, HZ., Bos, J.M., Gonzalez-Martinez, D., Vukmirovic, M., Turna, R.S., Sanchez-Gonzalez, M.A., Badger, C.D., Zorio, D.A., Singh, R.K., Wang, Y., Jin, J.P., Ackerman, M.J., Pinto, J.R. In vivo Analysis of Troponin C Knock-in (A8V) Mice: Evidence that TNNC1 Is a Hypertrophic Cardiomyopathy Susceptibility Gene. Circ Cardiovasc Genet. Oct;8(5):653-64, 2015

Chang, A.N., Battiprolu, P.K., Cowley, P.M., Chen, G., Gerard, R.D., Pinto, J.R., Hill, J.A., Baker, A.J., Kamm, K.E., Stull, J.T. Constitutive Phosphorylation of Cardiac Myosin Regulatory Light Chain in vivo. J. Biol. Chem. 290(17):10703-16, 2015

Parvatiyar, M.S. and Pinto, J.R. Pathogenesis Associated with a Restrictive Cardiomyopathy Mutant in Cardiac Troponin T is Due to Reduced Protein Stability and Greatly Increased Myofilament Ca2+ Sensitivity. Biochim. Biophys. Acta. Feb;1850(2):365-72, 2015

2014

Chang, A.N., Greenfield, N.J., Singh, A., Potter, J.D., Pinto, J.R. Structural and protein interaction effects of hypertrophic and dilated cardiomyopathic mutations in alpha-tropomyosin. Front. Physiol. Dec 2;5:460, 2014

Dweck, D., Sanchez-Gonzalez, M.A., Chang, A., Dulce, R., Badger, CD., Koutnik, A.P., Ruiz, E.L., Griffin, B., Liang, J., Kabbaj, M., Fincham, F., Hare, J., Overton, M.J., Pinto, J.R. Long term ablation of PKA-mediated cardiac troponin I phosphorylation leads to excitation-contraction uncoupling and diastolic dysfunction in a Knock-in mouse model of Hypertrophic Cardiomyopathy. J. Biol. Chem. 289:23097-23111, 2014

2013

Bai, F., Caster, H.M., Pinto, J.R., and Kawai, M. Functional properties of cardiomyopathy causing cTnT mutants I79N, DE96 and DK210: molecular pathogenesis study. Biophys. J. (London) 104(9): 1979-1988, 2013

Venkataraman, R., Baldo, M.P., Hwang, H., Veltri, T., Pinto, J.R., Baudenbacher, F.J., Knollmann, B.C. Myofilament calcium de-sensitization and contractile uncoupling with blebbistatin prevents ventricular tachycardia in mouse hearts with chronic myocardial infarction. J. Mol. Cell. Cardiol. 60C: 8-15, 2013

2012

Pinto, J.R., Gomes, A.V., Jones, M.A., Liang, J., Nguyen, S., Miller, T., Parvatiyar, M.S., and Potter, J.D. The Functional Properties of Human Slow Skeletal TnT Isoforms in the Cardiac Muscle Regulation. J. Biol. Chem. 287(44): 37362-70, 2012

Tao, G., Levay, A.K., Peacock, J.D., Huk, D.J., Both, S.N., Purcell, N.H., Pinto, J.R., Galantowicz, M.L., Koch, M., Lucchesi, P.A., Birk, D.E. and Lincoln J. Collagen XIV is important for growth and structural integrity of the myocardium. J. Mol. Cell. Cardiol. Nov;53(5):626-38, 2012

Parvatiyar, M., Landstrom, A., Figueiro-Freitas, C., Potter, J.D., Ackerman, M.J., Pinto, J.R. A mutation in TNNC1-encoded cardiac troponin C, TNNC1-A31S, predisposes to hypertrophic cardiomyopathy and ventricular fibrillation. J. Biol. Chem. 287(38):31845-31855, 2012

Ueta, C.B., Oskouei, B.N., Olivares, E.L., Pinto, J.R., Correa, M.M., Simovic, G., Simonides, W.S., Hare, J.M., Bianco, A.C. Absence of myocardial thyroid hormone inactivating deiodinase results in restrictive cardiomyopathy in mice. Mol Endocrinol. May;26(5): 809-18, 2012

Wang, Y., Pinto, J.R., Sancho Solis, R., Dweck, D., Liang, J., Diaz-Perez, Z., Ge, Y., Walker, J.W., Potter, J.D. The Generation and Functional Characterization of Knock-In Mice Harboring the Cardiac-troponin I R21C Mutation Associated with Hypertrophic Cardiomyopathy. J. Biol. Chem. 287(3): 2156-2167, 2012

2011

Pinto, J.R., Siegfried, J.D., Parvatiyar, M.S., Li, D., Norton, N., Jones, M.A., Liang, J., Potter, J.D., Hershberger, R.E. Functional Characterization of TNNC1 Rare Variants Identified in Dilated Cardiomyopathy. J. Biol. Chem. 286(39): 34404-34412, 2011

Midde, K., Dumka, V., Pinto, J.R., Muthu, P., Marandos, P., Gryczynski, I., Gryczynski, Z., Potter, J.D., Borejdo, J. Myosin cross-bridges do not form precise rigor bonds in hypertrophic heart muscle carrying troponin T mutations. J Mol Cell Cardiol. Sep;51(3): 409-418, 2011

Pinto, J.R., Yang, S.W., Hitz, M.P., Parvatiyar, M.S., Jones, M.A., Liang, J., Victor, K., Talajic, M., Tremblay, N., Jaeggi, M., Andelfinger, G. and Potter, J.D. Fetal Cardiac Troponin Isoforms Rescue the Increased Ca2+ Sensitivity Produced by a Novel Double Deletion in Cardiac Troponin T Linked to Restrictive Cardiomyopathy. A Clinical, Genetic and Functional Approach. J. Biol. Chem. 286(23): 20901-20912, 2011

Pinto, J.R., Sousa, V.P. and Sorenson, M.M. Redox State of Troponin C Cysteine in the D/E Helix Alters the C-domain Affinity for the Thin Filament of Vertebrate Striated Muscle. BBA - Gen Subj. 1810(4): 391-397, 2011

Pinto, J.R., Reynaldo, D.P., Parvatiyar, M.S., Dweck, D., Liang, J., Jones, M.A., Sorenson, M.M. and Potter, J.D. Strong Crossbridges Potentiate the Ca2+ Affinity Changes Produced by HCM-Cardiac Troponin C Mutants in Myofilaments. A Fast Kinetic Approach. J. Biol. Chem. 286(2): 1005-1013, 2011

2010

Morales, A., Pinto, J.R., Siegfried, J.D., Li, D., Norton, N., Hofmeyer, M., Vallin, M., Morales, A.R., Potter, J.D. and Hershberger, R.E. Late onset sporadic dilated cardiomyopathy caused by a cardiac troponin T mutation. Clin. Trans. Sci. 3:219-226, 2010

Parvatiyar, M.S., Pinto, J.R., Liang, J.S. and Potter, J.D. Predicting Cardiomyopathic Phenotypes by Altering the Ca2+ Affinity of Cardiac Troponin C. J. Biol. Chem. 285(36): 27785-97, 2010

Li, Y., Charles, P.Y.J., Nan, C., Pinto, J.R., Wang, Y., Liang, J., Wu, G., Tian, J., Feng, H.Z., Potter, J.D., Jin, J.P. and Huang, X. Correcting diastolic dysfunction by desensitizing troponin in transgenic RCM mice. J. Mol. Cell. Card. 49: 402-411, 2010

Dweck, D., Reynaldo, D.P., Pinto, J.R. and Potter, J.D. A Dilated Cardiomyopathy Troponin C Mutation Lowers Contractile Force by Reducing Strong Myosin-Actin Binding. J. Biol. Chem. 285(23): 17371-17379, 2010

Parvatiyar, M.S., Pinto, J.R., Dweck, D. and Potter, J.D. Cardiac Troponin Mutations and Restrictive Cardiomyopathy. J. Biomed. Biotechnol. ID 350706, 2010

Willott, R.W., Gomes, A.V., Parvatiyar, M.S., Chang, A., Pinto, J.R. and Potter, J.D. Mutation in Troponin that Cause Hypertrophic, Dilated and Restrictive Cardiomyopathy: What Can We Learn About Thin Filament Function? J. Mol. Cell Card. 48: 882-892, 2010

2009

Wen, Y., Xu, Y., Wang, Y., Pinto, J.R., Potter, J.D. and Kerrick, W.G.L. Functional Effects of a Restrictive Cardiomyopathy linked Cardiac Troponin I mutation (R145W) in Transgenic Mice. J. Mol. Biol., 392(5): 1158-1167, 2009

Pinto, J.R., Parvatiyar, M.S., Jones, M.A., Liang, J., Ackerman, M.J. and Potter, J.D. A functional and structural study of troponin C mutations related to hypertrophic cardiomyopathy. J. Biol. Chem, 284(28): 19090-19100, 2009

Hershberger, R.E., Pinto, J.R., Parks, S.B., Kushner, J.D., Li, D., Ludwigsen, S., Cowan, J., Morales, A., Parvatiyar, M.S. and Potter, J.D. Clinical and functional characterization of TNNT2 mutations identified in patients with dilated cardiomyopathy. Circulation: Cardiovasc. Genet., 2: 306-313, 2009

2008

Baudenbacher, F., Schober, T., Pinto, J.R., Sidorov, V., Hilliard, F., Solaro, R.J., Potter, J.D. and Knollmann, B.C. Myofilament Calcium Sensitization Causes Susceptibility to Cardiac Arrhythmia in Mice. J. Clin. Invest., 188(12): 3893-3903, 2008

Wen, Y., Pinto, J.R., Xu, Y., Wang, Y., Wang, Y., Gomes, A.V., Potter, J.D. and Kerrick, W.G.L. Functional Consequences of a Cardiac Troponin I (R145G) Mutation Associated with Hypertrophic Cardiomyopathy in Transgenic Mice. J. Biol. Chem., 283(29): 20484-20494, 2008

Landstrom, A.P.*, Parvatiyar, M.S.*, Pinto, J.R.*, Marquardt, M.L., Bos, J.M., Tester, D.J., Ommen, S.R., Potter, J.D. and Ackerman, M.J. Molecular and Functional Characterization of Novel Hypertrophic Cardiomyopathy Susceptibility Mutations in TNNC1- Encoded Troponin C. J. Mol. Cell Card., 45: 281-288, 2008 (*Co-first author)

Pinto, J.R., Veltri, T. and Sorenson, M.M. Modulation of Troponin C Affinity for the Thin Filament by Different Types of Crossbridges in Skinned Skeletal Muscle. Pflugers Archive, 456: 1177-1187, 2008

Pinto, J.R., Parvatiyar, M.S., Jones, M.A., Liang, J. and Potter, J.D. A Troponin T Mutation that Causes Infantile Restrictive Cardiomyopathy Increases Ca2+ Sensitivity of Force Development and Impairs the Inhibitory Properties of Troponin. J. Biol. Chem. 283: 2156-2166, 2008

2006

Sousa, V.P., Pinto, J.R. and Sorenson, M.M. Ionic Interventions that Alter the Association of Troponin C C-domain with the Thin Filaments of Vertebrate Striated Muscle. Biochim. Biophys. Acta (Gen. Subj.) 1760: 272-282, 2006

Braga, C.A.C.A., Pinto, J.R., Valente, A.P., Silva, J.L., Sorenson, M.M., Foguel, D. and Suarez, M.C. Ca2+ and Mg2+ Binding to the Weak Sites of TnC C-domain Induces Exposure of a Large Hydrophobic Surface that Leads to Loss of TnC from the Thin Filament. Intl. J. Biochem. Cell Biol. 38: 110-122, 2006

2004

Costa, L.T., Pinto, J.R., Moraes, M.B., Bastos, G.G., Sorenson, M.M., Bisch, P.M. and Weissmüller, G. Chemical Treatment of Mica for Atomic Force Microscopy Can Affect Biological Sample Conformation. Biophys. Chem. 109: 63-71, 2004