NovaSeq Data Access/Analysis
Information for Illumina NovaSeq 6000 sequencing data:
RCC Account:
In order to access the sequencing data, generate an account (guest account if outside FSU) with the FSU Research Computing Center (RCC).
Demultiplexing:
If sequencing samples are pooled and demultiplexing is needed, the user should provide an index sheet to TSL (yanming.yang@med.fsu.edu; cynthia.vied@med.fsu.edu). The index sheet should be generated using the Illumina Experiment Manager software for demultiplexing compatibility.
Data Access:
Data Transfer:
FSU RCC preferred approach for data transfer is via Globus.
Please use the following information on the Globus transfer page:
Endpoint: fsurcc#gpfs
Path to data: THE_PATH_RELEASED_VIA_EMAIL
Analysis Software:
The following software packages and others are available for data analysis at the RCC: FastQC, Samtools, Biopython, Abyss, Bowtie/Bowtie2, BWA, Star aligner, Tophat, Cufflinks, R packages, HTSeq, Trimmomatic. The software packages are available at general access and medicine spear nodes.