NovaSeq Data Access/Analysis

Information for Illumina NovaSeq 6000 sequencing data:

RCC Account:

In order to access the sequencing data, generate an account (guest account if outside FSU) with the FSU Research Computing Center (RCC).

Demultiplexing:

If sequencing samples are pooled and demultiplexing is needed, the user should provide an index sheet to TSL (yanming.yang@med.fsu.edu; cynthia.vied@med.fsu.edu). The index sheet should be generated using the Illumina Experiment Manager software for demultiplexing compatibility.

Data Access:

First, login to FSU RCC: YOUR_RCC_ID@hpc-login.rcc.fsu.edu.

Then, get to a spear node to manipulate your data:

Either:

ssh YOUR_RCC_ID@spear-X (X = 1,2,... 8) into general spear nodes (more powerful)

Or:

ssh YOUR_RCC_ID@spear-Y (Y = 17, 18, 19, 20, or 22) into medicine spear nodes

Data Transfer:

FSU RCC preferred approach for data transfer is via Globus.

Please use the following information on the Globus transfer page:
Endpoint: fsurcc#gpfs
Path to data: THE_PATH_RELEASED_VIA_EMAIL

Analysis Software:

The following software packages and others are available for data analysis at the RCC: FastQC, Samtools, Biopython, Abyss, Bowtie/Bowtie2, BWA, Star aligner, Tophat, Cufflinks, R packages, HTSeq, Trimmomatic. The software packages are available at general access and medicine spear nodes.