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Protein Biology Laboratory (PBL)

 

The Protein Biology Laboratory provides resources to study biomolecular interactions of proteins, nucleic acids, small molecules, and other cellular components. In addition, some aspects of molecular folding, protein conformation, and molecular stability can be determined.  These studies lend insights into fundamental aspects of molecular mechanisms and help decipher cellular regulatory processes in both healthy and diseased states.  As such, they facilitate translation of basic research into clinically relevant applications.  Some examples include studies aimed at development of therapeutic agents by examining interactions between a disease-associated protein and a drug candidate, or evaluating the structural stability of a potential therapeutic agent. 

The biophysical approaches utilized in the PBL include surface plasmon resonance (SPR), isothermal titration calorimetry (ITC), fluorescence, and UV-Vis spectroscopy. Consultation, training, and assistance in experimental design and data analysis are provided. 

The PBL capabilities include examination of:

  • Steady state binding affinity
  • Kinetics (association and dissociation constants)
  • Stoichiometry
  • Thermodynamics (enthalpy, entropy and free energy of binding)
  • Enzymatic parameters
  • Some aspects of molecular folding, conformation, and structural stability

 The PBL fee structure is as follows:

Resource Internal user fee External user fee Internal user training External user training
Surface plasmon resonance (SPR) $40/day $58.80/day $250/user $367.50/user
Isothermal titration calorimetry (ITC) $20/day $29.40/day $200/user $294/user

PBL Location:   College of Medicine Research Building, suite 3360 (East Bay). 

PBL Hours of operation:  8:00 am - 5 pm, Monday through Friday. Trained users have card access after hours.

Contact Information


COM 3350-J
Phone: (850) 645-7326
Fax: (850) 644-5781