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David Meckes Ph.D.

David Meckes Ph.D.

Assistant Professor

david.meckes@med.fsu.edu
(850) 645-2330
Main Campus

Job Description

Dr. Meckes is an Assistant Professor in the Department of Biomedical Sciences where he directs a research laboratory that is focused on understanding the molecular mechanisms of viral oncogenesis. Dr. Meckes also teaches Microbiology and facilitates small group sessions for medical students throughout the first two years of the curriculum.

Lab Web Page

Biosketch

Dr. Meckes obtained his B.S. from the University of the Sciences in Philadelphia and his Ph.D. from The Pennsylvania State University College of Medicine in the laboratory of Dr. John W. Wills. His dissertation research was focused on herpes simplex virus assembly and viral protein-protein interactions. Dr. Meckes completed his post-doctoral training in the laboratory of Dr. Nancy Raab-Traub at the University of North Carolina and was awarded an American Cancer Society fellowship to study the molecular properties of exosomes secreted from Epstein-Barr virus infected cancer cells. Dr. Meckes joined the Department of Biomedical Sciences at FSU in 2013.

Education

Postdoctoral training in Virology, University of North Carolina Lineberger Comprehensive Cancer Center (2009-2013)

Ph.D. in Microbiology and Immunology, The Pennsylvania State University College of Medicine (2008)

Bachelor of Science in Microbiology, University of the Sciences in Philadelphia (2003)

Service

2016 Curriculum Committee Review of Pre-clerkship Course
2015-present Curriculum Committee Year 1 and 2
2015-presnet By-Laws and Policy Committee
2015-present Faculty Recruitment Committee
2015 Graduate Program Committee
2015 Promotion and Tenure Committee
2013-present Curriculum Redesign Committees - Medicine 2, Host Defense, and Hematology
2014-present Speaker and organizer for BMS Public Information Session “Current Health Issues”
2014 Faculty Development and Mentoring Committee
2013-present Founder and member of FSU Virology Data Meeting

Honors/Awards

Outstanding Junior Faculty Investigator, FSU College of Medicine (2015)
Dr. Priscilla Shaffer award for top oral presentation by a postdoctoral fellow at the 37th Annual International Herpesvirus Workshop (2012)
Dr. Joseph S. Pagano Award for top paper by a postdoctoral fellow (2011)
American Cancer Society Postdoctoral Fellowship (2011-2013)
UNC Cancer Center Postdoctoral Training Fellowship from the NCI (2009-2011)
Enders’ Award for Scholarly Research Achievement, Penn State University (2008)
The 33rd International Herpesvirus Workshop Travel Award for top oral presentation (2008)
Predoctoral Training Fellowship: Viruses and Cancer, Penn State University (2006-2008)
Dr. Louis Gershenfeld Memorial Prize for Excellence in Microbiology (2003)
L. N. P. Rudolph Academic Scholarship (1999-2003)
Alpha Lambda Delta National Academic Honor Society (1999)

Memberships

International Society for Extracellular Vesicles
American Society for Exosomes and Microvesicles
American Society for Microbiology
International Association for Research on Epstein-Barr virus and Associated Diseases

Research Focus

Research Interests: Virus-host interactions, molecular pathogenesis of viral-associated cancers, signal transduction, extracellular vesicles, exosomes and intercellular communication

It is estimated that roughly 20 percent of all human cancers world-wide have a viral etiology or require virus infection as an essential cofactor. Epstein-Barr virus (EBV), a member of the human gamma herpesviruses, is associated with multiple human malignancies of both lymphoid and epithelial origin. EBV-associated cancers include Burkitt lymphoma, Hodgkin disease, nasopharyngeal carcinoma (NPC), and gastric carcinoma. EBV like all herpesviruses is characterized by its ability to persist for the lifetime of the host in a latent phase with sporadic reactivation and lytic replication. In EBV-associated cancers, the malignant cells contain the viral genome as an episome and express only a subset of viral genes. The latent membrane protein 1 (LMP1) of EBV is frequently detected in EBV-associated cancers and is considered the major oncogene of the virus. LMP1 is absolutely essential for EBV-induced B-cell immortalization and expression of LMP1 alone is sufficient to drive transformation of cells in vitro through the activation of cellular signaling pathways.

Recently, LMP1 has been found to be secreted from cancer cells in exosomes, small endocytically derived vesicles that participate in cell-cell communication. Data from our lab and others have shown that LMP1 modulates the functions and components of these vesicles. Exosomes are thought to function in many physiological processes through the transfer of biologically active proteins and RNAs between cells. Moreover, accumulating evidence suggests that alterations in normal exosome function may contribute to cancer pathogenesis. The research focus of the lab includes uncovering the mechanisms through which EBV modulates the host cell exosome pathway and determining how changes in this pathway may influence the tumor microenvironment and cancer progression. Additionally, we are interested in the molecular dissection of the cellular signal transduction pathways driving LMP1-induced transformation. To address these important questions in viral oncogenesis our laboratory employs cutting edge techniques in molecular biology, cell biology, biochemistry, and proteomics.

Publications

Google Scholar Profile

Research Gate Profile

Hurwitz, S.N., M.M. Conlon, M.A. Rider, D.G. Meckes, Jr. 2016. Nanoparticle analysis sheds budding insights into genetic drives of extracellular vesicle biogenesis. J. Extracell. Vesicles. 5:31295

Rider, M.A., S.N. Hurwitz, D.G. Meckes, Jr. 2016. ExtraPEG: A Polyethylene Glycol-Based Method for Enrichment of Extracellular Vesicles. Sci. Rep. 6:2397.

Meckes, D.G., Jr. 2015. Exosomal Communication Goes Viral. J Virol. 10:5200-3. Epub 2015 March 4

Meckes, D.G., Jr. (2014) Affinity purification combined with mass spectrometry to identify HSV protein-protein interactions. Methods Mol Biol. 1144:209-22.

Meckes, D.G., Jr., H. P. Gunawardena, R.M. Dekroon, P.R. Heaton, R. Hood Edwards, S. Ozgur, J.D. Griffith, B. Damania, N. Raab-Traub. (2013) Modulation of B-cell exosome proteins by gamma herpesvirus infection. Proc Natl Acad Sci USA. 110:2925-33.

Meckes, D.G., Jr. and N. Raab-Traub. Mining Epstein-Barr Virus LMP1 Signaling Networks. Jornal of Carcinogenesis and Mutagenesis. Special Issue on viral oncoproteomics. (in press)

Meckes, D. G., Jr., N. F. Menaker, and N. Raab-Traub (2013) Epstein-Barr virus LMP1 modulates lipid raft microdomains and the vimentin cytoskeleton for signal transduction and transformation. J Virol 87:1301-1311.

Meckes, D. G., Jr., and N. Raab-Traub (2011) Microvesicles and viral infection. J Virol 85:12844-12854.

Yeh, P. C., J. Han, P. Chadha, D. G. Meckes, Jr., M. D. Ward, O. J. Semmes, and J. W. Wills (2011) Direct and specific binding of the UL16 tegument protein of herpes simplex virus to the cytoplasmic tail of glycoprotein E. J Virol 85:9425-9436.

Han, J., P. Chadha, D. G. Meckes, Jr., N. L. Baird, and J. W. Wills (2011) Interaction and interdependent packaging of tegument protein UL11 and glycoprotein E of herpes simplex virus. J Virol 85:9437-9446.

Kung, C. P.*, D. G. Meckes, Jr.*, and N. Raab-Traub (2011) Epstein-Barr virus LMP1 activates EGFR, STAT3, and ERK through effects on PKCdelta. J Virol 85:4399-4408. *These authors contributed equally to this work.

Meckes, D. G., Jr., K. H. Shair, A. R. Marquitz, C. P. Kung, R. H. Edwards, and N. Raab-Traub (2010) Human tumor virus utilizes exosomes for intercellular communication. Proc Natl Acad Sci USA 107:20370-20375.

Meckes, D. G., Jr., J. A. Marsh, and J. W. Wills (2010) Complex mechanisms for the packaging of the UL16 tegument protein into herpes simplex virus. Virology 398:208-213.

Harper, A. L.*, D. G. Meckes, Jr.*, J. A. Marsh*, M. D. Ward, P. C. Yeh, N. L. Baird, C. B. Wilson, O. J. Semmes, and J. W. Wills (2010) Interaction domains of the UL16 and UL21 tegument proteins of herpes simplex virus. J Virol 84:2963-2971. *These authors contributed equally to this work.

Meckes, D. G., Jr., and J. W. Wills (2008) Structural rearrangement within an enveloped virus upon binding to the host cell. J Virol 82:10429-10435.

Yeh, P. C., D. G. Meckes, Jr., and J. W. Wills (2008) Analysis of the interaction between the UL11 and UL16 tegument proteins of herpes simplex virus. J Virol 82:10693-10700.

Meckes, D. G., Jr., and J. W. Wills (2007) Dynamic interactions of the UL16 tegument protein with the capsid of herpes simplex virus. J Virol 81:13028-13036.